The shares were bought from the Susli family, which owned the Praktiker chain, Kingfisher said in a statement Wednesday.
The financial aspects of the deal have not been disclosed and the deal is subject to antitrust clearance.
Kingfisher has 15 Brico Depôt stores in Romania and 900 employees. The retail home improvement group has over 1,200 stores in ten European countries.
Praktiker is one of the main player on the Romanian retail home improvement market, with 27 stores in Romania. It had a turnover of around EUR140 million in 2016.